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Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190

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Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190

品牌：RevMAb

貨號：31-1058-00

規格：100 ug

貨期：電詢

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Product Details
Product Name	Anti-Histone H3.3 Rabbit Monoclonal Antibody [RM190]
Product Description	Rabbit monoclonal to Histone H3.3
Catalog No.	31-1058-00
Clone Name	RM190
Product Data Sheet	Anti-Histone H3.3 Rabbit Monoclonal Antibody Data Sheet RM190
Specificity	RM190 reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
Immunogen	A peptide corresponding to human Histone H3.3.
Application	Western Blot (WB) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
.
Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
References for Histone H3.3 Rabbit Monoclonal Antibody [RM190]	Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation, Nat Cell Biol., 10.1038/ncb3348. (2016) IF; Human, Monkey. Read more (PubMed: 27136267)
Product Images
	RM190 reacts specifically to Histone H3.3. No cross reactivity with Histone H3.1.
	Western Blot of HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using RM190 at 1 ug/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1.