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CD4 Antibody (PE)

品牌：Abbexa

貨號：abx134642

規格：50 Assays

貨期：

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CD4 Antibody (PE)

商品詳情參考文獻相關資料

Mouse monoclonal antibody to CD4 (PE).

Target	CD4
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Tested Applications	IF/ICC, FCM
Purification	The antibody was purified by affinity chromatography.
Form	Liquid
Isotype	IgG_2a
Conjugation	PE
Specificity	Recognises human CD4.
Storage	Shipped and store at 4°C for one year. Do not freeze.
Swiss Prot	P01730
GeneID	920
Gene Symbol	CD4
Buffer	PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Directions for use	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of a 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of the flow tube mixing with the whole blood; incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes away from light after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.