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Based on N-terminal sequencing, the mature recombinant IL-28A starts at Val 26 and has a calculated molecular mass of 20.8 kDa. As a result of glycosylation, the recombinant monomer migrates as an approximately 24 kDa protein in SDS-PAGE under reducing conditions.

DNA sequence encoding human IL-28A with a carboxyl-terminal polyhistidine tag expressed in mouse myeloma cell line, NSO

Greater than 95% as determined by a combination of HPLC methods
Endotoxin level < 1 EU/μg

Human HepG2 cells infected with encephalomyocarditis virus, ED50 ~10-50 ng/ml

Phosphate buffered saline (PBS)

< 1 EU/μg

For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles